MOLECULAR CHARACTERIZATION OF INFECTIOUS BURSAL DISEASE VIRUS FROM COMMERCIAL POULTRY IN PAKISTAN

Incidence and Molecular Characterization of Infectious Bursal Disease Virus in Commercial Broilers in Pakistan

The study was conducted to determine the prevalence of infectious bursal disease virus (IBDV) at different ages in commercial boilers. From Oct 2003 to Nov 2004, bursa samples collected from both vaccinated as well non-vaccinated commercially reared broiler chickens suspected of having infectious bursal disease were analyzed for the presence of IBDV using reverse transcriptase polymerase chain ...

Characterization of a Highly Virulent Infectious Bursal Disease Virus

Molecular Typing of Field Isolates from two outbreaks of Infectious Bursal Disease Virus from Pakistan

A reverse transcriptase polymerase chain reaction restriction fragment length polymorphism (RT-PCR/RFLP) technique was used for the identification and characterization of Pakistani field isolates of infectious bursal disease virus (IBDV). A total of 8 bursa samples were collected from two outbreaks during September and October 2003 from Tehsil Sumandri, Dist. Faisalabad with 40-50% mortality in...

Isolation, Characterization and Standardization of New Infectious Bursal Disease Virus for Development of a Live Vaccine

Background and Aims: Infectious bursal disease (IBD) is an acute contagious viral disease of birds worldwide. The causative virus induces a persistent immune suppression following destroy B lymphocytes precursors in bursal lymphoid follicles. Vaccination is the main strategy for prevention of the disease in commercial poultry industry. Materials and Methods: To produce a live vaccine against th...

Molecular characterization of field infectious bursal disease virus isolates from Nigeria

AIM To characterize field isolates of infectious bursal disease virus (IBDV) from outbreaks in nine states in Nigeria through reverse transcription polymerase chain reaction (RT-PCR) and sequence analysis of portions of the VP2 and VP1 genes and to determine the presence or absence of reassortant viruses. MATERIALS AND METHODS A total of 377 bursa samples were collected from 201 suspected IBD...